33 7 70 9 AMES ASSAY FOR MUTAGENICITY
18 11 84 12 Diethyl 3, 3-Dimethyl-2-oxo-1, 4-cyclopentanedicarboxylate
10 11 16 13 COMPOUND
15 14 49 16 Lorillard - Organic Chemistry
62 14 67 15 A123
83 14 92 15 OR67-13
10 15 15 16 SOURCE
52 15 60 16 LORILLARD NO:
78 15 82 16 LOT NO:
23 17 31 19 unknown
45 17 62 19 3/18/81-4/8/81
74 17 83 19 4/14/81
10 18 19 19 DATE RECEIVED
41 18 45 19 TESTED
64 18 70 19 REPORTED
20 20 49 22 H. S. Tong & A. A. Poole
79 20 87 22 BC19-25
10 21 19 22 INVESTIGATOR(S)
68 21 78 23 NOTEBOOK PAGE
22 22 43 27 
54 22 73 25 
10 24 18 25 SIGNATURE(S):
10 29 22 31 TOXICITY (%) (SURVIVAL)
36 30 38 31 50
45 30 46 31 80
62 30 67 31 SOLVENT
68 30 70 31 ☑
53 30 55 31 100
71 30 74 31 DMSO
75 30 81 31 ☐ WATER
10 32 23 33 CONCENTRATION mg/ml
43 33 47 34 .025
69 33 75 34 ☐ OTHER
10 37 19 38 S. TYPHIMURIUM
35 37 39 38 TA1535
51 37 55 38 TA100
67 37 70 38 TA98
32 38 35 39 (-)S-9
39 38 43 39 (+)S-9
47 38 51 39 (-)S-9
55 39 58 40 (+)S-9
63 39 67 40 (-)S-9
71 39 74 40 (+)S-9
79 39 83 40 (-)S-9
87 39 90 40 (+)S-9
10 40 25 42 CONTROL REVERTANTS PER PLATE TOON MI SOLVENTI
31 41 35 43 9.67
38 42 44 43 14.00
45 42 52 43 118.00
53 42 60 43 127.00
62 42 67 43 9.00
69 42 76 43 21.00
10 45 21 46 COMPOUND plates
40 45 80 46 REVERSION RATE TEST REVERTANTS-CONTROL REVERTANTS PER PLATE)
10 47 13 48 25
40 47 44 49 .76
57 47 60 49 .87
72 47 75 49 .65
10 49 15 51 12.5
31 49 36 51 1.10
39 49 44 51 1.12
47 50 52 51 1.07
57 50 60 51 .82
64 50 67 51 .78
72 50 75 51 .71
10 52 15 53 6.25
40 52 44 53 .52
57 52 60 53 .88
72 52 75 53 .86
10 58 20 59 POSTIVE CONTROL (ug plate)
10 61 21 62 2. AMINOANTHRACENE
10 63 13 64 4.0
32 63 35 64 .72
39 63 44 64 3.07
48 63 52 64 .98
55 63 60 64 4.26
63 63 67 64 .22
69 63 75 64 20.10
10 65 14 66 2.0
31 65 35 66 1.24
39 65 43 66 2.86
48 65 52 66 .76
55 65 60 67 3.63
64 65 67 66 .67
69 65 75 67 18.81
10 67 26 69 Streptozotocin
10 69 13 70 1.0
31 69 36 71 6.41
38 69 43 71 31.14
47 69 52 71 1.60
55 69 60 71 3.11
10 71 14 73 0.5
31 71 35 73 2.28
38 71 43 73 12.29
55 71 60 73 1.99
47 72 52 73 1.08
18 78 85 80 This compound is judged non - mutagenic in this test system.
9 79 16 80 CONCLUSION
94 82 96 91 01073843
10 83 38 84 Copies to the following:
40 83 59 85 H. J. Minnemeyer
40 85 52 87 L. B. Gray
9 94 26 95 LORILLARD RESEARCH CENTER
85 95 92 96 FORM 580
