84 1 90 3 
33 7 69 9 AMES ASSAY FOR MUTAGENICITY
18 11 43 13 2, 4- Dihydroxypyridine
9 11 15 13 COMPOUND
61 14 68 16 OR61 -2
17 14 51 16 Lorillard - Organic Chemistry
9 15 14 16 SOURCE:
51 15 57 16 LORILLARD
77 15 80 16 LOT
57 15 59 16 NO
80 15 82 16 NO.
46 17 65 19 9 /10 /80 -10 /10 /80
78 17 87 19 10 /10 /80
22 17 29 19 9 /3 /80
40 18 44 20 TESTED
9 18 18 19 DATE RECEIVED
63 18 70 19 REPORTED:
22 20 50 22 H. S. Tong & A. A. Poole
80 20 88 22 BIO7 -24
9 21 18 22 INVESTIGATOR(S)
68 21 77 23 NOTEBOOK PAGE
23 22 77 26 
9 24 17 25 SIGNATURE(S)
9 30 22 31 TOXICITY (% SURVIVAL)
61 30 66 31 SOLVENT
70 30 73 31 DMSO
36 30 37 31 50
52 30 55 31 100
68 30 70 31 ☑
74 30 81 31 ☐ WATER
44 30 45 31 80
9 32 22 34 CONCENTRATION 1mgimit
68 32 74 34 ☐ OTHER
44 33 46 34 .5
9 37 18 38 S. TYPHIMURIUM
35 37 39 38 TA1535
51 37 54 38 TA100
67 37 70 38 TA98
31 38 35 39 (-)S-9
39 38 42 40 (+)S-9
47 38 50 40 (-)S-9
55 39 58 40 (+)S-9
63 39 66 40 (-)S-9
70 39 74 40 (+)S-9
79 39 82 40 (-)S-9
87 39 90 40 (+)S-9
9 40 24 42 CONTROL AFVERTANTS PER PLATE (0.05 ml SOLVENT)
38 41 43 43 8.00
30 41 35 43 4.67
45 41 52 43 123.00
53 41 61 43 135.33
69 42 75 43 16.00
63 42 67 43 3.33
9 45 20 46 COMPOUND (US plate)
39 45 80 47 REVERSION RATE (TEST REVERSANTS/CONTROL REVERTANTS PER PLATE
39 47 43 49 .92
17 47 21 49 500
57 47 60 49 .88
71 47 75 49 .96
49 49 52 51 .97
31 49 36 51 1.00
64 49 67 51 .90
40 49 43 51 .79
17 50 20 51 250
57 50 60 51 .97
71 50 75 51 .92
17 52 21 53 125
40 52 43 53 .83
57 52 61 53 .92
71 52 75 53 .85
9 58 19 60 POSTIVE CONTROL (ug plate)
9 61 21 62 2- AMINOANTHHACENE
31 63 36 65 1.20
38 63 43 65 8.63
63 63 68 65 1.80
46 63 52 65 1.39
17 63 19 64 4
55 63 60 64 7.26
70 63 76 64 27.69
64 65 68 67 .30
70 65 76 67 24.13
17 65 19 67 2
38 65 44 67 4.75
46 65 51 67 1.24
55 65 60 67 6.07
33 65 36 67 .86
21 78 90 80 This compound is judged non mutagenic in this test system.
9 79 16 81 CONCLUSION
92 80 94 90 00836244
17 83 45 85 Copies to the following:
47 83 71 87 Dr. H. J. Minnemeyer Ms. L. B. Gray
9 94 26 96 LORILLARD RESEARCH CENTER
85 94 92 96 FORM (5/80)
