Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Coordinated poleward flux of sister kinetochore fibers drives chromosome alignment Chromosome alignment at the spindle equator promotes proper chromosome segregation and depends on pulling forces exerted at kinetochore fiber tips together with polar ejection forces. However, kinetochore fibers are also subjected to forces driving their poleward flux. Here we introduce a flux-driven centering model that relies on flux generated by forces within the overlaps of bridging and kinetochore fibers. This centering mechanism works so that the longer kinetochore fiber fluxes faster than the shorter one, moving the kinetochores towards the center. We developed speckle microscopy in human spindles and confirmed the key prediction that kinetochore fiber flux is length-dependent. Kinetochores are better centered when overlaps are shorter and the kinetochore fiber flux markedly slower than the bridging fiber flux. We identify Kif18A and Kif4A as overlap and flux regulators and NuMA as a fiber coupler. Thus, length-dependent sliding forces exerted by the bridging fiber onto kinetochore fibers promote chromosome alignment.
11
The categories of this paper are: cognition
10
The categories of this paper are: and
31
The categories of this paper are: paleontology
29
The categories of this paper are: education
13
The categories of this paper are: animal
23
The categories of this paper are: immunology
24
The categories of this paper are: cancer
22
The categories of this paper are: physiology
27
The categories of this paper are: pathology
28
The categories of this paper are: communication
30
The categories of this paper are: scientific
9
The categories of this paper are: synthetic
17
The categories of this paper are: molecular
1
The categories of this paper are: bioinformatics
6
The categories of this paper are: plant
32
The categories of this paper are: zoology