Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: A multi-state dynamical process underpins mechano-adaptation in the bacterial flagellar motor Adaptation is a defining feature of living systems. The bacterial flagellar motor adapts to changes in the external mechanical environment by adding or removing torque-generating stator units. However, the molecular mechanism for mechanosensitive motor remodeling remains unclear. Here, we induced stator disassembly using electrorotation, followed by the time-dependent assembly of the individual stator units into the motor after electrorotation was terminated. From these experiments, we extracted detailed statistics of the dwell times that comprise the stochastic dynamics of the binding and unbinding of stator units. Dwell times reveal multiple timescales, indicating the existence of multiple binding states of the stator units. Based on these results, we propose a minimal model in which the stator unit can occupy four different states - two bound states with very different rates of unbinding, a diffusive unbound state, and a transiently detached state. Our minimal model quantitatively explains multiple features of the experimental data and allows us to determine the transition rates among all four states. Our experiments and modeling suggest a mechanism of mechano-adaptive remodeling of the bacterial flagellar motor in which torque generated by bound stator units controls their effective unbinding rate by modulating the transition between the two bound states. Furthermore, the binding rate of stator units with the motor has a non-monotonic dependence on the number of bound units, likely because of two counteracting effects of motor rotation on the binding process.
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The categories of this paper are: neuroscience
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The categories of this paper are: toxicology
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The categories of this paper are: bioinformatics
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The categories of this paper are: cognition
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The categories of this paper are: plant
31
The categories of this paper are: paleontology
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The categories of this paper are: genetics
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The categories of this paper are: cancer
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The categories of this paper are: zoology
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The categories of this paper are: and
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The categories of this paper are: physiology
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The categories of this paper are: pharmacology
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The categories of this paper are: systems
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The categories of this paper are: education
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The categories of this paper are: developmental
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The categories of this paper are: molecular