Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: In-cell structures of a conserved supramolecular array at the mitochondria-cytoskeleton interface in mammalian sperm Mitochondria-cytoskeleton interactions modulate cellular physiology by regulating mitochondrial transport, positioning, and immobilization. However, there is very little structural information defining mitochondria-cytoskeleton interfaces in any cell type. Here, we use cryo-focused ion beam milling-enabled cryo-electron tomography to image mammalian sperm, where mitochondria wrap around the ciliary cytoskeleton. We find that mitochondria are tethered to their neighbors through inter-mitochondrial linkers and are anchored to the cytoskeleton through ordered arrays on the outer mitochondrial membrane. We use subtomogram averaging to resolve in-cell structures of these arrays from three mammalian species, revealing they are conserved across species despite variations in mitochondrial dimensions and cristae organization. We find that the arrays consist of boat-shaped particles anchored on a network of membrane pores whose arrangement and dimensions are consistent with voltage dependent anion channels. Proteomics and in-cell cross-linking mass spectrometry suggest that the conserved arrays are composed of glycerol kinase-like proteins. Ordered supramolecular assemblies may serve to stabilize similar contact sites in other cell types where mitochondria need to be immobilized in specific subcellular environments, such as in muscles and neurons.
14
The categories of this paper are: biophysics
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The categories of this paper are: synthetic
27
The categories of this paper are: pathology
25
The categories of this paper are: bioengineering
0
The categories of this paper are: genetics
18
The categories of this paper are: systems
32
The categories of this paper are: zoology
6
The categories of this paper are: plant
4
The categories of this paper are: neuroscience
21
The categories of this paper are: toxicology
7
The categories of this paper are: ecology
30
The categories of this paper are: scientific
11
The categories of this paper are: cognition
13
The categories of this paper are: animal
1
The categories of this paper are: bioinformatics
22
The categories of this paper are: physiology