Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Chondroitin sulfates enhances the barrier function of basement membrane assembled by heparan sulfates Glycosaminoglycans (GAGs) are ubiquitously expressed polysaccharides attached to proteoglycans, but their functions in the retina are poorly understood. Here we generated conditional knockouts of biosynthetic enzymes for heparan sulfate (HS) and chondroitin sulfate (CS) in retinal progenitor cells. We showed that ablation of HS polymerase Ext1 did not affect initial progression of retinal angiogenesis, but it disrupted the pruning of blood vessels and establishment of arterioles and venules. In the absence of retinal HS, blood vessels were also vulnerable to high oxygen tension in early postnatal stages, which can be rescued by exogenous VEGF, consistent with the role of retinal HS in the fine-tuning of VEGF signaling. Furthermore, we observed that the retinal inner limiting membrane (ILM) was disrupted by deletion of Ext1 in a timing specific manner, suggesting that retinal HS is required for the assembly but not the maintenance of the ILM. Lastly, we showed that further deletion of C4st1, a CS sulfation enzyme, did not affect the assembly of the ILM, but aggravated the ILM permeability when combined with Ext1 deletion. These results demonstrated an important role of CS and HS in establishing the barrier function of basement membrane.
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The categories of this paper are: neuroscience
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The categories of this paper are: microbiology
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The categories of this paper are: physiology
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The categories of this paper are: scientific
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The categories of this paper are: bioengineering
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The categories of this paper are: plant
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The categories of this paper are: cancer
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The categories of this paper are: education
31
The categories of this paper are: paleontology
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The categories of this paper are: behavior
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The categories of this paper are: biophysics
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The categories of this paper are: cell
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The categories of this paper are: biochemistry
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The categories of this paper are: and
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The categories of this paper are: pathology
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The categories of this paper are: systems