Instruct: Identify the main and secondary category of Arxiv papers based on the titles and abstracts
Query: Pressure and temperature dependence of fluorescence anisotropy of Green
  Fluorescent Protein   We have studied the effect of high hydrostatic pressure and temperature on
the steady state fluorescence anisotropy of Green Fluorescent Protein (GFP). We
find that the fluorescence anisotropy of GFP at a constant temperature
decreases with increasing pressure. At atmospheric pressure, anisotropy
decreases with increasing temperature but exhibits a maximum with temperature
for pressure larger than 20 MPa. The temperature corresponding to the maximum
of anisotropy increases with increasing pressure. By taking into account of the
rotational correlation time changes of GFP with the pressure-temperature
dependent viscosity of the solvent, we argue that viscosity increase with
pressure is not a major contributing factor to the decrease in anisotropy with
pressure. Our results suggest that the decrease of fluorescence anisotropy with
pressure may result from changes in H-bonding environment around the
chromophore.

18
The categories of this paper are: econ
12
The categories of this paper are: nucl-th
33
The categories of this paper are: plasm-ph
35
The categories of this paper are: mtrl-th
8
The categories of this paper are: hep-ex
13
The categories of this paper are: quant-ph
17
The categories of this paper are: q-fin
3
The categories of this paper are: physics
31
The categories of this paper are: ao-sci
28
The categories of this paper are: alg-geom
34
The categories of this paper are: chem-ph
14
The categories of this paper are: math-ph
37
The categories of this paper are: supr-con
29
The categories of this paper are: funct-an
16
The categories of this paper are: stat
1
The categories of this paper are: cs