Instruct: Identify the main and secondary category of Arxiv papers based on the titles and abstracts
Query: Importance of method validation: Implications of non-correlated
  observables in sweet taste receptor studies   Validation of research methodology is critical in research design.
Correlation between experimental observables must be established before
undertaking extensive experiments or propose mechanisms. This article shows
that, observables in the popular calcium flux strength assay used in the
characterization of sweetener-sweet taste receptor (STR) interaction are
uncorrelated. In pursuit to find potential sweeteners and enhancers, calcium
flux generated via G-protein coupling for wildtype and mutant receptors
expressed on cell surface is measured to identify and localize sweetener
binding sites. Results are channeled for sweetener development with direct
impact on public health.
  We show that flux strength is independent of EC50 and sweet potency. Sweet
potency-EC50 relation is non-linear and anti-correlated. Single point mutants
affecting receptor efficiency, without significant shift in EC50 have been
published, indicating flux strength is independent of ligand binding. G-protein
coupling step is likely observed in the assay. Thus, years have been spent
generating uncorrelated data. Data from uncorrelated observables does not give
meaningful results. Still, majority of research in the field, uses change in
calcium flux strength to study the receptor. Methodology away from flux
strength monitor is required for sweetener development, reestablish binding
localization of sweeteners established by flux strength method. This article
serves to remind researchers to validate methodology before plunging into long
term projects. Ignoring validation test on methodology, have been a costly
mistake in the field. Concepts discussed here is applicable, whenever
observable in biological systems are many steps moved from the event of
interest.

26
The categories of this paper are: bayes-an
18
The categories of this paper are: econ
2
The categories of this paper are: math
36
The categories of this paper are: cmp-lg
20
The categories of this paper are: acc-phys
31
The categories of this paper are: ao-sci
29
The categories of this paper are: funct-an
7
The categories of this paper are: hep-th
28
The categories of this paper are: alg-geom
30
The categories of this paper are: q-alg
3
The categories of this paper are: physics
35
The categories of this paper are: mtrl-th
11
The categories of this paper are: hep-lat
14
The categories of this paper are: math-ph
27
The categories of this paper are: comp-gas
9
The categories of this paper are: nlin