Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Genes with 5' terminal oligopyrimidine tracts preferentially escape global suppression of translation by the SARS-CoV-2 NSP1 protein Viruses rely on the host translation machinery to synthesize their own proteins. Consequently, they have evolved varied mechanisms to co-opt host translation for their survival. SARS-CoV-2 relies on a non-structural protein, Nsp1, for shutting down host translation. However, it is currently unknown how viral proteins and host factors critical for viral replication can escape a global shutdown of host translation. Here, using a novel FACS-based assay called MeTAFlow, we report a dose-dependent reduction in both nascent protein synthesis and mRNA abundance in cells expressing Nsp1. We perform RNA-Seq and matched ribosome profiling experiments to identify gene-specific changes both at the mRNA expression and translation level. We discover a functionally-coherent subset of human genes are preferentially translated in the context of Nsp1 expression. These genes include the translation machinery components, RNA binding proteins, and others important for viral pathogenicity. Importantly, we uncovered a remarkable enrichment of 5' terminal oligo-pyrimidine (TOP) tracts among preferentially translated genes. Using reporter assays, we validated that 5 UTRs from TOP transcripts can drive preferential expression in the presence of NSP1. Finally, we found that LARP1, a key effector protein in the mTOR pathway may contribute to preferential translation of TOP transcripts in response to Nsp1 expression. Collectively, our study suggests fine tuning of host gene expression and translation by Nsp1 despite its global repressive effect on host protein synthesis.
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The categories of this paper are: toxicology
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The categories of this paper are: scientific
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The categories of this paper are: systems
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The categories of this paper are: cancer
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The categories of this paper are: microbiology
28
The categories of this paper are: communication
14
The categories of this paper are: biophysics
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The categories of this paper are: pathology
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The categories of this paper are: pharmacology
3
The categories of this paper are: evolutionary
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The categories of this paper are: immunology
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The categories of this paper are: synthetic
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The categories of this paper are: developmental
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The categories of this paper are: genetics
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The categories of this paper are: biochemistry
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The categories of this paper are: behavior