Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: The Digital 3D-Atlas MAKER (DAMAKER): a dynamic and expandable digital 3D-tool for monitoring the temporal changes in tissue growth during hindbrain morphogenesis Reconstruction of prototypic three-dimensional (3D) atlases at the scale of whole tissues or organs requires specific methods to be developed. We have established a digital 3D-atlas maker (DAMAKER) and built a digital 3D-temporal atlas to monitor the changes in the growth of the tissue and neuronal differentiation domain in the zebrafish hindbrain. DAMAKER integrates spatial and temporal data from cell populations, neuronal differentiation and brain morphogenesis, through in vivo imaging techniques paired with image analyses and segmentation tools. First, we generated a 3D-reference from several imaged hindbrains and segmented them using a trainable tool; these were aligned using rigid registration, revealing distribution of neuronal differentiation growth patterns along the axes. Second, we quantified the dynamic growth of the neuronal differentiation domain vs. the progenitor domain, and by in vivo neuronal birthdating experiments we generated a digital 3D-temporal map of the neuronal growth in the whole hindbrain, revealing the spatiotemporal dynamics of neuronal differentiation upon morphogenesis. Last, we applied it to glutamatergic and GABAergic neurons, as proof-of-concept that the digital 3D-temporal map could be used as a proxy to infer neuronal birthdate. As this protocol uses open-access tools and algorithms, it can be shared for standardized, accessible, tissue-wide cell population atlas construction.
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The categories of this paper are: behavior
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The categories of this paper are: genomics
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The categories of this paper are: molecular
31
The categories of this paper are: paleontology
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The categories of this paper are: pharmacology
30
The categories of this paper are: scientific
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The categories of this paper are: cell
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The categories of this paper are: animal
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The categories of this paper are: toxicology
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The categories of this paper are: evolutionary
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The categories of this paper are: genetics
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The categories of this paper are: education
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The categories of this paper are: neuroscience
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The categories of this paper are: synthetic
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The categories of this paper are: cognition
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The categories of this paper are: ecology