Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Capsaicin pretreatment alleviates postoperative pain and reduces primary sensory neuron Ca2+ activity After surgeries, especially thoracotomy incision, patients develop unbearable pain. Opioids are used for reducing pain but often cause serious side effects. Previously, we found that capsaicin pretreatment of the incision area alleviated spontaneous and thermal pain in a postoperative pain animal model. In the present study, we aimed to monitor primary sensory neuron Ca2+ activity in in vivo dorsal root ganglia (DRG) in a postoperative pain model using Pirt-GCaMP3 treated with capsaicin or controls. Intraplantar injection of capsaicin (0.05%) alleviated spontaneous, mechanical, and thermal postoperative pain. The Ca2+ response in in vivo DRG and in in situ spinal cord was significantly enhanced in the ipsilateral side compared to contralateral side or naive control. Primary sensory nerve fiber length was significantly decreased in the incision skin area in capsaicin-pretreated animals detected by immunohistochemistry and placental alkaline phosphatase (PLAP) staining. Thus, capsaicin pretreatment alleviates postoperative pain by suppressing Ca2+ response due to degeneration of primary sensory nerve fibers in the skin.
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The categories of this paper are: physiology
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The categories of this paper are: genetics
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The categories of this paper are: communication
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The categories of this paper are: biology
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The categories of this paper are: and
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The categories of this paper are: toxicology
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The categories of this paper are: microbiology
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The categories of this paper are: bioengineering
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The categories of this paper are: biophysics
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The categories of this paper are: plant
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The categories of this paper are: developmental
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The categories of this paper are: immunology
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The categories of this paper are: cell
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The categories of this paper are: synthetic
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The categories of this paper are: systems
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The categories of this paper are: ecology