Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Nascent adhesions differentially regulate lamellipodium velocity and persistence Cell migration is essential to physiological and pathological biology. Migration is driven by the motion of a leading edge, in which actin polymerization pushes against the edge and adhesions transmit traction to the substrate while membrane tension increases. How the actin and adhesions synergistically control edge protrusion remains elusive. We addressed this question by developing a computational model in which the Brownian ratchet mechanism governs actin filament polymerization against the membrane and the molecular clutch mechanism governs adhesion to the substrate (BR-MC model). Our model predicted that actin polymerization is the most significant driver of protrusion, as actin had a greater effect on protrusion than adhesion assembly. Increasing the lifetime of nascent adhesions also enhanced velocity, but decreased the protrusions motional persistence, because filaments maintained against the cell edge ceased polymerizing as membrane tension increased. We confirmed the model predictions with measurement of adhesion lifetime and edge motion in migrating cells. Adhesions with longer lifetime were associated with faster protrusion velocity and shorter persistence. Experimentally increasing adhesion lifetime increased velocity but decreased persistence. We propose a mechanism for actin polymerization-driven, adhesion-dependent protrusion in which balanced nascent adhesion assembly and lifetime generates protrusions with the power and persistence to drive migration.
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The categories of this paper are: neuroscience
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The categories of this paper are: plant
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The categories of this paper are: behavior
22
The categories of this paper are: physiology
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The categories of this paper are: ecology
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The categories of this paper are: pathology
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The categories of this paper are: genetics
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The categories of this paper are: and
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The categories of this paper are: genomics
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The categories of this paper are: animal
28
The categories of this paper are: communication
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The categories of this paper are: zoology
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The categories of this paper are: bioengineering
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The categories of this paper are: toxicology
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The categories of this paper are: molecular
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The categories of this paper are: systems