Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Binary outcomes of enhancer activity underlie stable random monoallelic expression Mitotically stable random monoallelic gene expression (RME) is documented for a small percentage of autosomal genes. Here we investigated the role of enhancers in the RME of natural killer (NK) cell receptor genes. Enhancers were accessible and enriched in H3K27ac on silent and active alleles alike, decoupling enhancer activation and expression. Enhancers controlled gene expression frequency, as predicted by the binary model of enhancer action, and enhancer deletion converted the broadly expressed Nkg2d into an RME gene, recapitulating natural variegation. The results suggested that RME is a consequence of general enhancer properties and therefore many genes may be subject to some degree of RME, which was borne out by analysis of a panel of genes previously thought to be universally expressed within defined hematopoietic lineages: Nkg2d, Cd45, Cd8a and Thy1. We propose that previously documented RME is an extreme on a continuum of intrinsically probabilistic gene expression.
23
The categories of this paper are: immunology
9
The categories of this paper are: synthetic
14
The categories of this paper are: biophysics
5
The categories of this paper are: cell
0
The categories of this paper are: genetics
25
The categories of this paper are: bioengineering
8
The categories of this paper are: genomics
10
The categories of this paper are: and
22
The categories of this paper are: physiology
26
The categories of this paper are: biochemistry
24
The categories of this paper are: cancer
29
The categories of this paper are: education
11
The categories of this paper are: cognition
7
The categories of this paper are: ecology
3
The categories of this paper are: evolutionary
19
The categories of this paper are: epidemiology