Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: The Rab11-Family Interacting Proteins reveal selective interaction of mammalian recycling endosomes with the Toxoplasma parasitophorous vacuole in a Rab11- and Arf6-dependent manner After invasion of mammalian cells, the parasite Toxoplasma gondii multiplies in a self-made membrane-bound compartment, the parasitophorous vacuole (PV). We previously showed that intravacuolar Toxoplasma interacts with many host cell organelles, especially recycling endosomes, and further manipulates the host endocytic recycling through the sequestration of Rab11 vesicles into the PV. Mammalian Rab-PV interactions are likely mediated by Toxoplasma and host proteins that remain to be identified. In this context, we have examined the specificity of host Rab vesicle interaction with the PV by monitoring the recruitment of subtypes of Rab11 vesicles differing in their composition in Rab11-Family Interacting Proteins (FIPs). We found that vesicles with FIPs from Class I (FIP1C, FIP2, FIP5) or Class II (FIP3, FIP4) are distributed at the PV and detected to varying degrees inside the PV. The PV delivery of vesicles with FIPs from Class I, but not Class II, is Rab11-dependent. In addition to Rab11, FIP3 binds to Arf6, and vesicles associated with FIP3-Arf6 complexes are observed within the PV. Binding of FIP3 to either Rab11 or Arf6 significantly increases the internalization of vesicles into the PV. These data point to a selective process of host recycling endosome recognition and scavenging mediated by Toxoplasma.
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The categories of this paper are: and
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The categories of this paper are: behavior
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The categories of this paper are: ecology
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The categories of this paper are: synthetic
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The categories of this paper are: genetics
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The categories of this paper are: communication
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The categories of this paper are: cognition
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The categories of this paper are: pharmacology
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The categories of this paper are: bioinformatics
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The categories of this paper are: molecular
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The categories of this paper are: immunology
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The categories of this paper are: zoology
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The categories of this paper are: plant
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The categories of this paper are: systems
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The categories of this paper are: toxicology
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The categories of this paper are: developmental