Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Characterization of the composition and functioning of the Crumbs complex in  C. elegans The apical domain of epithelial cells can acquire a diverse array of morphologies and functions, which is critical for the function of epithelial tissues. The Crumbs proteins are evolutionary conserved transmembrane proteins with essential roles in promoting apical domain formation in epithelial cells. The short intracellular tail of Crumbs proteins interacts with a variety of proteins, including the scaffolding protein Pals1 (protein associated with LIN7, Stardust in Drosophila). Pals1 in turn binds to a second scaffolding protein termed PATJ (Pals1-associated tight junction protein), to form the core Crumbs/Pals1/PATJ Crumbs complex. While essential roles in epithelial organization have been shown for Crumbs proteins in Drosophila and mammalian systems, the three Caenorhabditis elegans crumbs genes are dispensable for epithelial polarization and animal development. Moreover, the presence and functioning of orthologs of Pals1 and PATJ has not been investigated. Here, we identify MAGU-2 and MPZ-1 as the C. elegans orthologs of Pals1 and PATJ, respectively. We show that MAGU-2 interacts with all three Crumbs proteins as well as MPZ-1, and localizes to the apical membrane domain in a Crumbs-dependent fashion. Similar to crumbs mutants, a magu-2 null mutant shows no developmental or epithelial polarity defects. Finally, we show that overexpression of the Crumbs proteins EAT-20 or CRB-3 in the C. elegans intestine can lead to apical membrane expansion. Our results shed light into the composition of the C. elegans Crumbs complex and indicate that the role of Crumbs proteins in promoting apical domain formation is conserved.
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The categories of this paper are: scientific
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The categories of this paper are: and
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The categories of this paper are: biophysics
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The categories of this paper are: behavior
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The categories of this paper are: genetics
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The categories of this paper are: cognition
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The categories of this paper are: paleontology
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The categories of this paper are: molecular
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The categories of this paper are: evolutionary
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The categories of this paper are: bioengineering
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The categories of this paper are: cell
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The categories of this paper are: epidemiology
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The categories of this paper are: education
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The categories of this paper are: toxicology
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The categories of this paper are: immunology
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The categories of this paper are: plant