Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Multi-omics analysis of glucose-mediated signaling by a moonlighting Gβ protein Asc1/RACK1 G proteins were originally discovered through efforts to understand the effects of hormones, such as glucagon and epinephrine, on glucose metabolism. On the other hand, many cellular metabolites, including glucose, serve as ligands for G protein-coupled receptors. Here we investigate the consequences of glucose-mediated receptor signaling, and in particular the role of a G subunit Gpa2 and a non-canonical G{beta} subunit, known as Asc1 in yeast and RACK1 in animals. Asc1/RACK1 is of particular interest because it has multiple, seemingly unrelated, functions in the cell. The existence of such "moonlighting" operations has complicated the determination of phenotype from genotype. Through a comparative analysis of individual gene deletion mutants, and by integrating transcriptomics and metabolomics measurements, we have determined the relative contributions of the G and G{beta} protein subunits to glucose-initiated processes in yeast. We determined that Gpa2 is primarily involved in regulating sugar metabolism while Asc1 is primarily involved in amino acid metabolism. Both proteins are involved in regulating purine metabolism. Of the two subunits, Gpa2 regulates a greater number of gene transcripts and was particularly important in determining the amplitude of response to glucose addition. We conclude that the two G protein subunits regulate distinct but complementary processes downstream of the glucose-sensing receptor, as well as processes that lead ultimately to changes in cell growth and metabolism.

AUTHOR SUMMARYDespite the societal importance of glucose fermentation in yeast, the mechanisms by which these cells detect and respond to glucose have remained obscure. Glucose detection requires a cell surface receptor coupled to a G protein that is comprised of two subunits, rather than the more typical heterotrimer: an  subunit Gpa2 and the {beta} subunit Asc1 (or RACK1 in humans). Asc1/RACK1 also serves as a subunit of the ribosome, where it regulates the synthesis of proteins involved in glucose fermentation. This manuscript uses global metabolomics and transcriptomics to demonstrate the distinct roles of each G protein subunit in transmitting the glucose signal. Whereas Gpa2 is primarily involved in the metabolism of sugars, Asc1/RACK1 contributes to production of amino acids necessary for protein synthesis and cell division. These findings reveal the initial steps of glucose signaling and several unique and complementary functions of the G protein subunits. More broadly, the integrated approach used here is likely to guide efforts to determine the topology of complex G protein and metabolic signaling networks in humans.
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The categories of this paper are: education
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The categories of this paper are: communication
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The categories of this paper are: cognition
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The categories of this paper are: cell
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The categories of this paper are: scientific
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The categories of this paper are: toxicology
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The categories of this paper are: paleontology
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The categories of this paper are: genetics
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The categories of this paper are: biochemistry
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The categories of this paper are: evolutionary
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The categories of this paper are: molecular
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The categories of this paper are: and
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The categories of this paper are: bioinformatics
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The categories of this paper are: ecology
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The categories of this paper are: systems
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The categories of this paper are: animal