Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Identification of unknown proteins in X-ray crystallography and cryo-EM Although experimental protein structure determination usually targets known proteins, chains of unknown sequence are often encountered. They can be purified from natural sources, appear as an unexpected fragment of a well characterized protein or as a contaminant. Regardless of the source of the problem, the unknown protein always requires tedious characterization. Here we present an automated pipeline for the identification of protein sequences from cryo-EM reconstructions and crystallographic data. We present the methods application to characterize the crystal structure of an unknown protein purified from a snake venom. We also show that the approach can be successfully applied to the identification of protein sequences and validation of sequence assignments in cryo-EM protein structures.
14
The categories of this paper are: biophysics
24
The categories of this paper are: cancer
32
The categories of this paper are: zoology
18
The categories of this paper are: systems
30
The categories of this paper are: scientific
26
The categories of this paper are: biochemistry
13
The categories of this paper are: animal
19
The categories of this paper are: epidemiology
0
The categories of this paper are: genetics
25
The categories of this paper are: bioengineering
4
The categories of this paper are: neuroscience
11
The categories of this paper are: cognition
8
The categories of this paper are: genomics
6
The categories of this paper are: plant
23
The categories of this paper are: immunology
3
The categories of this paper are: evolutionary