Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Visualizing Molecular Interactions that Determine Assembly of a Bullet-Shaped Vesicular Stomatitis Virus Particle Vesicular stomatitis virus (VSV) is a negative-strand RNA virus with a non-segmented genome, closely related to rabies virus. Both have characteristic bullet-like shapes. We report the structure of intact, infectious VSV particles determined by cryogenic electron microscopy. By compensating for polymorphism among viral particles with computational classification, we obtained a reconstruction of the shaft ("trunk") at 3.5 [A] resolution, with lower resolution for the rounded tip. The ribonucleoprotein (RNP), genomic RNA complexed with nucleoprotein (N), curls into a dome-like structure with about eight gradually expanding turns before transitioning into the regular helical trunk. Two layers of matrix (M) protein link the RNP with the membrane. Radial inter-layer subunit contacts are fixed within single RNA-N-M1-M2 modules, but flexible lateral and axial interactions allow assembly of polymorphic virions. Together with published structures of recombinant N in various states, our results suggest a mechanism for membrane- coupled self-assembly of VSV and its relatives.
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The categories of this paper are: physiology
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The categories of this paper are: neuroscience
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The categories of this paper are: developmental
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The categories of this paper are: animal
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The categories of this paper are: epidemiology
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The categories of this paper are: behavior
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The categories of this paper are: cell
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The categories of this paper are: synthetic
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The categories of this paper are: biochemistry
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The categories of this paper are: pharmacology
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The categories of this paper are: toxicology
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The categories of this paper are: paleontology
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The categories of this paper are: and
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The categories of this paper are: pathology
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The categories of this paper are: ecology
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The categories of this paper are: education