Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Transcriptome profiling reveals CD73 and age-driven changes in neutrophil responses against Streptococcus pneumoniae Neutrophils are required for host resistance against Streptococcus pneumoniae but their function declines with age. We previously found that CD73, an enzyme required for antimicrobial activity, is down-regulated in neutrophils from aged mice. This study explored transcriptional changes in neutrophils induced by S. pneumoniae to identify pathways controlled by CD73 and dysregulated with age. Ultrapure bone marrow-derived neutrophils isolated from wild type (WT) young, old, and CD73KO young mice were mock-challenged or infected with S. pneumoniae ex vivo. RNA sequencing was performed to identify differentially expressed genes (DEGs). We found that infection triggered distinct global transcriptional changes across hosts, that were strongest in CD73KO neutrophils. Surprisingly, there were more down-regulated than up-regulated genes in all groups upon infection. Down-regulated DEGs indicated a dampening of immune responses in old and CD73KO hosts. Further analysis revealed that CD73KO neutrophils expressed higher numbers of long non-coding RNAs (lncRNAs) compared to WT controls. Predicted network analysis indicated that CD73KO specific lncRNAs control several signaling pathways. We found that genes in the JNK-MAPK-pathway were up-regulated upon infection in CD73KO and WT old but not in young mice. This corresponded to functional differences, as phosphorylation of the downstream AP-1 transcription factor component c-Jun was significantly higher in infected CD73KO and old mice neutrophils. Importantly, inhibiting JNK/AP-1 rescued the ability of these neutrophils to kill S. pneumoniae. Altogether, our findings revealed that neutrophils modify their gene expression to better adapt to bacterial infection and that this capacity declines with age and is regulated by CD73.
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The categories of this paper are: plant
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The categories of this paper are: ecology
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The categories of this paper are: zoology
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The categories of this paper are: molecular
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The categories of this paper are: synthetic
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The categories of this paper are: genetics
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The categories of this paper are: cell
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The categories of this paper are: paleontology
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The categories of this paper are: biology
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The categories of this paper are: communication
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The categories of this paper are: cancer
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The categories of this paper are: biochemistry
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The categories of this paper are: developmental
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The categories of this paper are: pathology
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The categories of this paper are: neuroscience
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The categories of this paper are: pharmacology