Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Defining the activation profile and fate trajectory of adult Scleraxis-lineage cells during tendon healing by combining lineage tracing and spatial transcriptomics The tendon healing process is regulated by the coordinated interaction of multiple cell types and molecular processes. However, these processes are not well-defined leading to a paucity of therapeutic approaches to enhance tendon healing. Scleraxis-lineage (ScxLin) cells are the major cellular component of adult tendon and make time-dependent contributions to the healing process. Prior work from our lab and others suggests heterogeneity within the broader ScxLin population over the course of tendon healing; therefore delineating the temporal and spatial contributions of these cells is critical to understanding and improving the healing process. In the present study we utilize lineage tracing of the adult aScxLin population to determine whether these cells undergo cellular activation and subsequent myofibroblast differentiation, which is associated with both proper healing and fibrotic progression in many tissues. We show that adult aScxLin cells undergo transient activation in the organized cellular bridge at the tendon repair site, contribute to the formation of an organized neotendon, and contribute to a persistent myofibroblast population in the native tendon stubs. The mechanisms dictating this highly specialized spatial response are unknown. We therefore utilized spatial transcriptomics to better define the spatio-molecular program of tendon healing. Integrated transcriptomic analyses across the healing time-course identifies five distinct molecular regions, including key interactions between the inflammatory bridging tissue and highly reactive tendon tissue at the repair site, with adult ScxLin cells being a central player in the transition from native tendon to reactive, remodeling tendon. Collectively, these data provide important insights into both the role of adult ScxLin cells during healing as well as the molecular mechanisms that underpin and coordinate the temporal and spatial healing phenotype, which can be leveraged to enhance the healing process.
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The categories of this paper are: pharmacology
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The categories of this paper are: neuroscience
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The categories of this paper are: bioengineering
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The categories of this paper are: biophysics
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The categories of this paper are: developmental
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The categories of this paper are: and
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The categories of this paper are: cancer
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The categories of this paper are: microbiology
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The categories of this paper are: plant
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The categories of this paper are: genetics
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The categories of this paper are: physiology
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The categories of this paper are: education
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The categories of this paper are: synthetic
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The categories of this paper are: toxicology
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The categories of this paper are: paleontology
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The categories of this paper are: cell