Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Structural and functional characterization of NEMO cleavage by SARS-CoV-2 3CLpro In addition to its essential role in viral polyprotein processing, the SARS-CoV-2 3C-like (3CLpro) protease can cleave human immune signaling proteins, like NF-{kappa}B Essential Modulator (NEMO) and deregulate the host immune response. Here, in vitro assays show that SARS-CoV-2 3CLpro cleaves NEMO with fine-tuned efficiency. Analysis of the 2.14 [A] resolution crystal structure of 3CLpro C145S bound to NEMO226-235 reveals subsites that tolerate a range of viral and host substrates through main chain hydrogen bonds while also enforcing specificity using side chain hydrogen bonds and hydrophobic contacts. Machine learning- and physics-based computational methods predict that variation in key binding residues of 3CLpro- NEMO helps explain the high fitness of SARS-CoV-2 in humans. We posit that cleavage of NEMO is an important piece of information to be accounted for in the pathology of COVID-19.
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The categories of this paper are: cognition
21
The categories of this paper are: toxicology
0
The categories of this paper are: genetics
32
The categories of this paper are: zoology
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The categories of this paper are: behavior
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The categories of this paper are: pathology
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The categories of this paper are: plant
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The categories of this paper are: developmental
22
The categories of this paper are: physiology
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The categories of this paper are: animal
3
The categories of this paper are: evolutionary
24
The categories of this paper are: cancer
5
The categories of this paper are: cell
25
The categories of this paper are: bioengineering
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The categories of this paper are: systems
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The categories of this paper are: pharmacology