Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Cytoplasmic processing of human transfer RNAs Biogenesis of different classes of eukaryotic RNAs proceeds via different pathways that require strict spatiotemporal resolution. The intracellular organization has evolved to provide order for RNA processing to coordinate different maturation steps with specific enzymatic reaction. In higher eukaryotes, processing of transfer RNAs (tRNAs) is postulated to be almost entirely intranuclear, while in lower eukaryotes like yeast, tRNA maturation is both nuclear and cytoplasmic. Here, we show that tRNA processing is largely cytosolic event in human cells. After transcription, unprocessed precursors of tRNAs (pre-tRNAs) bound by La protein are exported from the nucleus into the cytoplasm. Using cell fractionation analysis and pre-tRNA-specific fluorescence in situ hybridization (FISH) protocol, we show tRNA splicing/ligation and end-processing takes place in the cytoplasm where majority of processing enzymes is also located. We propose here a model where processing of intron-less and intron-containing pre-tRNAs is cytoplasmic similarly to the observed in lower eukaryotes, although tRNA splicing precedes 5- and 3-end processing in human cells unlike in yeast.
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The categories of this paper are: animal
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The categories of this paper are: epidemiology
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The categories of this paper are: physiology
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The categories of this paper are: genomics
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The categories of this paper are: systems
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The categories of this paper are: toxicology
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The categories of this paper are: synthetic
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The categories of this paper are: and
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The categories of this paper are: zoology
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The categories of this paper are: developmental
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The categories of this paper are: scientific
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The categories of this paper are: behavior
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The categories of this paper are: genetics
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The categories of this paper are: bioengineering
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The categories of this paper are: microbiology
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The categories of this paper are: bioinformatics