Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Cellular resolution anatomical and molecular atlases for prenatal human brains Increasing interest in studies of prenatal human brain development, particularly using new single-cell genomics and anatomical technologies to create cell atlases, creates a strong need for accurate and detailed anatomical reference atlases. In this study, we present two cellular-resolution digital anatomical atlases for prenatal human brain at post-conceptional weeks (PCW) 15 and 21. Both atlases were annotated on sequential Nissl-stained sections covering brain-wide structures on the basis of combined analysis of cytoarchitecture, acetylcholinesterase staining and an extensive marker gene expression dataset. This high information content dataset allowed reliable and accurate demarcation of developing cortical and subcortical structures and their subdivisions. Furthermore, using the anatomical atlases as a guide, spatial expression of 37 and 5 genes from the brains respectively at PCW 15 and 21 was annotated, illustrating reliable marker genes for many developing brain structures. Finally, the present study uncovered several novel developmental features, such as the lack of an outer subventricular zone in the hippocampal formation and entorhinal cortex, and the apparent extension of both cortical (excitatory) and subcortical (inhibitory) progenitors into the prenatal olfactory bulb. These comprehensive atlases provide useful tools for visualization, targeting, imaging and interpretation of brain structures of prenatal human brain, and for guiding and interpreting the next generation of cell census and connectome studies.
27
The categories of this paper are: pathology
7
The categories of this paper are: ecology
32
The categories of this paper are: zoology
15
The categories of this paper are: developmental
13
The categories of this paper are: animal
25
The categories of this paper are: bioengineering
1
The categories of this paper are: bioinformatics
10
The categories of this paper are: and
26
The categories of this paper are: biochemistry
31
The categories of this paper are: paleontology
24
The categories of this paper are: cancer
17
The categories of this paper are: molecular
19
The categories of this paper are: epidemiology
22
The categories of this paper are: physiology
16
The categories of this paper are: microbiology
5
The categories of this paper are: cell