Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Contribution of the Wolffian duct mesenchyme to the formation of the female reproductive tract The female reproductive tract develops from its embryonic precursor, the Mullerian duct.

In close proximity to the Mullerian duct lies the precursor for the male reproductive tract, the Wolffian duct, which is eliminated in the female embryo during sexual differentiation. We discovered that a component of the Wolffian duct, its mesenchyme, is not eliminated after sexual differentiation. Instead, the Wolffian duct mesenchyme underwent changes in transcriptome and chromatin accessibility from male tract to female tract identity, and became a unique mesenchymal population in the female reproductive tract with localization and transcriptome distinct from the mesenchyme derived from the Mullerian duct. Partial ablation of the Wolffian duct mesenchyme stunted the growth of the fetal female reproductive tract. These findings reveal a new fetal origin of mesenchymal tissues for female reproductive tract formation and reshape our understanding of sexual differentiation of reproductive tracts.

Significance statementA female embryo initially possesses both the primitive female and male reproductive tracts, also known as the Mullerian and Wolffian duct. During sexual differentiation, the female eliminates the Wolffian duct and maintains the Mullerian duct that eventually differentiates into the female reproductive tract organs. However, in this paper, we show the female embryo retains mesenchymal cells surrounding the Wolffian duct for its reproductive tract formation. When incorporated into the female reproductive tract organs, the Wolffian duct mesenchyme shows unique anatomical localization and transcriptome and plays critical roles in female reproductive tract growth. This discovery provides new insights into female reproductive tract development and advances our understanding of sexual differentiation of reproductive tracts.
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The categories of this paper are: cancer
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The categories of this paper are: pathology
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The categories of this paper are: immunology
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The categories of this paper are: biophysics
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The categories of this paper are: scientific
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The categories of this paper are: epidemiology
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The categories of this paper are: pharmacology
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The categories of this paper are: bioengineering
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The categories of this paper are: zoology
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The categories of this paper are: neuroscience
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The categories of this paper are: synthetic
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The categories of this paper are: animal
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The categories of this paper are: bioinformatics
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The categories of this paper are: cognition
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The categories of this paper are: plant
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The categories of this paper are: ecology