Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Multifunctional properties of Nej1XLF C-terminus promote end-joining and impact DNA double-strand break repair pathway choice A DNA double strand break (DSB) is primarily repaired by one of two canonical pathways, non-homologous end-joining (NHEJ) and homologous recombination (HR). NHEJ requires no or minimal end processing for ligation, whereas HR requires 5 end resection followed by a search for homology. The main event that determines the mode of repair is the initiation of 5 resection because if resection starts, then NHEJ cannot occur. Nej1 is a canonical NHEJ factor that functions at the cross-roads of repair pathway choice and prior to its function in stimulating Dnl4 ligase. Nej1 competes with Dna2, inhibiting its recruitment to DSBs and thereby inhibiting resection. The highly conserved C-terminal region (CTR) of Nej1 (330-338) is important for two events that drive NHEJ, stimulating ligation and inhibiting resection, but it is dispensable for end-bridging. By combining nej1 point mutants with nuclease-dead dna2-1, we find that Nej1-F335 is essential for end-joining whereas V338 promotes NHEJ indirectly through inhibiting Dna2-mediated resection.

HighlightsO_LINej1 C-terminus is critical for repair pathway choice.
C_LIO_LIThe KKRK region of Nej1 is important for interactions with ssDNA and dsDNA.
C_LIO_LINej1-F335 and V338 are key residues for end-joining and inhibition of resection at DSB.
C_LIO_LINej1-mediated end-bridging is not sufficient for end-joining repair.
C_LI
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The categories of this paper are: toxicology
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The categories of this paper are: paleontology
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The categories of this paper are: cancer
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The categories of this paper are: and
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The categories of this paper are: cognition
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The categories of this paper are: biophysics
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The categories of this paper are: immunology
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The categories of this paper are: plant
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The categories of this paper are: scientific
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The categories of this paper are: behavior
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The categories of this paper are: bioengineering
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The categories of this paper are: neuroscience
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The categories of this paper are: genetics
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The categories of this paper are: synthetic
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The categories of this paper are: microbiology
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The categories of this paper are: physiology