Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Root membrane ubiquitinome under short-term osmotic stress Osmotic stress can be detrimental to plants, whose survival relies heavily on proteomic plasticity. Protein ubiquitination is a central post-translational modification in osmotic mediated stress. Plants use the ubiquitin (Ub) proteasome system to modulate protein content, and a role for Ub in mediating endocytosis and trafficking plant plasma membrane proteins has recently emerged. In this study, we used the K-{varepsilon}-GG antibody enrichment method integrated with high-resolution mass spectrometry to compile a list of 719 ubiquitinated lysine (K-Ub) residues from 450 Arabidopsis root membrane proteins (58% of which are transmembrane proteins), thereby adding to the database of ubiquitinated substrates in plants. Although no Ub motifs could be identified, the presence of acidic residues close to K-Ub was revealed. Our ubiquitinome analysis pointed to a broad role of ubiquitination in the internalization and sorting of cargo proteins. Moreover, the simultaneous proteome and ubiquitinome quantification showed that ubiquitination is mostly not involved in membrane protein degradation in response to short osmotic treatment, but putatively in protein internalization as described for the aquaporin PIP2;1. Our in silico analysis of ubiquitinated proteins shows that two E2 Ub ligases, UBC32 and UBC34, putatively target membrane proteins under osmotic stress. Finally, we revealed a positive role for UBC32 and UBC34 in primary root growth under osmotic stress.
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The categories of this paper are: bioengineering
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The categories of this paper are: physiology
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The categories of this paper are: animal
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The categories of this paper are: genomics
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The categories of this paper are: cancer
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The categories of this paper are: genetics
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The categories of this paper are: neuroscience
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The categories of this paper are: epidemiology
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The categories of this paper are: toxicology
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The categories of this paper are: behavior
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The categories of this paper are: evolutionary
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The categories of this paper are: education
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The categories of this paper are: communication
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The categories of this paper are: immunology
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The categories of this paper are: developmental
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The categories of this paper are: biochemistry