Instruct: Identify the main category of Medrxiv papers based on the titles and abstracts
Query: Bicentric evaluation of stabilizing sampling tubes for assessment of monocyte HLA-DR expression in clinical samples BackgroundDiminished expression of human leukocyte antigen DR on circulating monocytes (mHLA-DR), measured by standardized flow cytometry procedure, is a reliable indicator of immunosuppression in severely injured intensive care unit patients. As such, it is used as stratification criteria in clinical trials evaluating novel immunostimulating therapies. Pre-analytical constraints relative to the short delay between blood sampling and flow cytometry staining have nevertheless limited its use in multicentric studies. The objective of the present work was to compare mHLA-DR expression between whole blood samples simultaneously drawn in EDTA or Cyto-Chex(R) BCT tubes.

MethodsIn 2 university hospitals, mHLA-DR was assessed in fresh whole blood from septic patients (n = 12) and healthy donors (n = 6) simultaneously sampled on EDTA and Cyto-Chex(R) BCT tubes. Staining was performed immediately after sampling and after blood storage at room temperature.

ResultsWe observed the remarkable stability of mHLA-DR results when blood was collected in Cyto-Chex(R) BCT tubes (until 48-72 h). On baseline values, despite good correlation between tubes (r = 0.98, p< 0.001), mHLA-DR expression was systematically lower with Cyto-Chex(R) BCT.

ConclusionThe present reports confirms the great potential of Cyto-Chex(R) BCT tubes to delay mHLA-DR staining in centers without rapid access to flow cytometry facilities. However, a 30 % gap exists between results obtained with EDTA and Cyto-Chex(R) BCT tubes. As current thresholds for clinical decisions were obtained with EDTA samples, further studies are needed to confirm clinical thresholds with Cyto-Chex(R) BCT tubes.
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The categories of this paper are: genetic
5
The categories of this paper are: health
67
The categories of this paper are: transplantation
20
The categories of this paper are: psychiatry
57
The categories of this paper are: primary
61
The categories of this paper are: dermatology
55
The categories of this paper are: obstetrics
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The categories of this paper are: policy
65
The categories of this paper are: oral
72
The categories of this paper are: urology
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The categories of this paper are: infectious
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The categories of this paper are: therapy
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The categories of this paper are: dentistry
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The categories of this paper are: global
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The categories of this paper are: surgery
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The categories of this paper are: sexual