Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Generation of rat lungs by blastocyst complementation in Fgfr2b-deficient mouse model Regenerative medicine is a tool to compensate for the shortage of lungs for transplantation, but it remains difficult to construct a lung in vitro due to the complex three-dimensional structures and multiple cell types required. A blastocyst complementation method using interspecies chimeric animals has been attracting attention as a way to create complex organs in animals, but successful lung formation has not yet been achieved. Here, we applied a "reverse-blastocyst complementation method" to clarify the conditions required to form lungs in an Fgfr2b-deficient mouse model. We then successfully formed a rat-derived lung in the mouse model without generating a mouse line by applying a tetraploid-based organ-complementation method. Importantly, rat lung epithelial cells retained their developmental timing even in the mouse body. This result provides useful insights regarding the need to overcome the barrier of species-specific developmental timing in order to generate functional lungs in interspecies chimeras.
16
The categories of this paper are: microbiology
32
The categories of this paper are: zoology
14
The categories of this paper are: biophysics
8
The categories of this paper are: genomics
12
The categories of this paper are: behavior
28
The categories of this paper are: communication
18
The categories of this paper are: systems
22
The categories of this paper are: physiology
23
The categories of this paper are: immunology
1
The categories of this paper are: bioinformatics
25
The categories of this paper are: bioengineering
31
The categories of this paper are: paleontology
17
The categories of this paper are: molecular
0
The categories of this paper are: genetics
11
The categories of this paper are: cognition
5
The categories of this paper are: cell