Instruct: Identify the main and secondary category of Arxiv papers based on the titles and abstracts
Query: Effects of excitation light polarization on fluorescence emission in
  two-photon light-sheet microscopy   Light-sheet microscopy (LSM) is a powerful imaging technique that uses a
planar illumination oriented orthogonally to the detection axis. Two-photon
(2P) LSM is a variant of LSM that exploits the 2P absorption effect for sample
excitation. The light polarization state plays a significant, and often
overlooked, role in 2P absorption processes. The scope of this work is to test
whether using different polarization states for excitation light can affect the
detected signal levels in 2P LSM imaging of typical biological samples with a
spatially unordered dye population. Supported by a theoretical model, we
compared the fluorescence signals obtained using different polarization states
with various fluorophores (fluorescein, EGFP and GCaMP6s) and different samples
(liquid solution and fixed or living zebrafish larvae). In all conditions, in
agreement with our theoretical expectations, linear polarization oriented
parallel to the detection plane provided the largest signal levels, while
perpendicularly-oriented polarization gave low fluorescence signal with the
biological samples, but a large signal for the fluorescein solution. Finally,
circular polarization generally provided lower signal levels. These results
highlight the importance of controlling the light polarization state in 2P LSM
of biological samples. Furthermore, this characterization represents a useful
guide to choose the best light polarization state when maximization of signal
levels is needed, e.g. in high-speed 2P LSM.

8
The categories of this paper are: hep-ex
2
The categories of this paper are: math
17
The categories of this paper are: q-fin
14
The categories of this paper are: math-ph
7
The categories of this paper are: hep-th
31
The categories of this paper are: ao-sci
11
The categories of this paper are: hep-lat
9
The categories of this paper are: nlin
1
The categories of this paper are: cs
16
The categories of this paper are: stat
19
The categories of this paper are: eess
36
The categories of this paper are: cmp-lg
20
The categories of this paper are: acc-phys
0
The categories of this paper are: hep-ph
30
The categories of this paper are: q-alg
32
The categories of this paper are: atom-ph