Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Systematic discovery of receptor-ligand biology by engineered cell entry and single-cell genomics Cells communicate with each other via receptor-ligand interactions on the cell surface. Here we describe a technology for lentiviral-mediated cell entry by engineered receptor-ligand interaction (ENTER) to decode receptor specificity. Engineered lentiviral particles displaying specific ligands deliver fluorescent proteins into target cells upon cognate receptor-ligand interaction, without genome integration or transgene transcription. We optimize ENTER to decode interactions between T cell receptor (TCR)-MHC peptides, antibody-antigen, and other receptor-ligand pairs. We develop an effective presentation strategy to capture interactions between B cell receptor (BCR) and intracellular antigen epitopes. Single-cell readout of ENTER by RNA sequencing (ENTER-seq) enables multiplexed enumeration of TCR-antigen specificities, clonality, cell type, and cell states of individual T cells. ENTER-seq of patient blood samples after CMV infection reveals the viral epitopes that drive human effector memory T cell differentiation and inter-clonal phenotypic diversity that targets the same epitope. ENTER enables systematic discovery of receptor specificity, linkage to cell fates, and cell-specific delivery of gene or protein payloads.

HIGHLIGHTSO_LIENTER displays ligands, deliver cargos, and records receptor specificity.
C_LIO_LIENTER deorphanizes antigen recognition of TCR and BCR.
C_LIO_LIENTER-seq maps TCR specificity, clonality and cell state in single cells.
C_LIO_LIENTER-seq of patient sample decodes antiviral T cell memory.
C_LI
26
The categories of this paper are: biochemistry
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The categories of this paper are: cancer
25
The categories of this paper are: bioengineering
28
The categories of this paper are: communication
29
The categories of this paper are: education
3
The categories of this paper are: evolutionary
23
The categories of this paper are: immunology
17
The categories of this paper are: molecular
0
The categories of this paper are: genetics
15
The categories of this paper are: developmental
14
The categories of this paper are: biophysics
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The categories of this paper are: cognition
19
The categories of this paper are: epidemiology
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The categories of this paper are: paleontology
27
The categories of this paper are: pathology
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The categories of this paper are: animal