Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: The CTPase activity of ParB acts as a timing mechanism to control the dynamics and function of prokaryotic DNA partition complexes DNA partitioning CTPases of the ParB family mediate the segregation of bacterial chromosomes and low-copy number plasmids. They act as DNA-sliding clamps that are loaded at parS motifs in the centromeric region of target DNA molecules and then spread laterally to form large nucleoprotein complexes that serve as docking points for the DNA segregation machinery. Here, we identify conformational changes that underlie the CTP- and parS-dependent closure of ParB clamps. Moreover, we solve crystal structures of ParB in the pre- and post-hydrolysis state and provide insights into the catalytic mechanism underlying nucleotide hydrolysis. The characterization of CTPase-deficient ParB variants reveals that CTP hydrolysis serves as a timing mechanism to control the sliding time of ParB. Hyperstable clamps are trapped on the DNA, leading to excessing spreading and severe chromosome segregation defects in vivo. These findings clarify the role of the ParB CTPase cycle in partition complex dynamics and function and thus complete our understanding of this prototypic CTP-dependent molecular switch.
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The categories of this paper are: epidemiology
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The categories of this paper are: scientific
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The categories of this paper are: pathology
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The categories of this paper are: cognition
28
The categories of this paper are: communication
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The categories of this paper are: physiology
5
The categories of this paper are: cell
31
The categories of this paper are: paleontology
14
The categories of this paper are: biophysics
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The categories of this paper are: pharmacology
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The categories of this paper are: plant
32
The categories of this paper are: zoology
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The categories of this paper are: bioengineering
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The categories of this paper are: animal
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The categories of this paper are: neuroscience
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The categories of this paper are: biology