Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: HSP90-CDC37-PP5 forms a structural platform for kinase dephosphorylation Activation of client protein kinases by the HSP90 molecular chaperone system is affected by phosphorylation at multiple sites on HSP90, on the kinase specific co-chaperone CDC37, and the kinase client itself. Removal of regulatory phosphorylation from client kinases and their release from the HSP90-CDC37 system depends on a Ser/Thr phosphatase PP5, which associates with HSP90 via its N-terminal TPR domain. Here we present the cryoEM structure of the oncogenic protein kinase client BRAFV600E bound to HSP90-CDC37, showing how the V600E mutation favours BRAF association with HSP90-CDC37. Structures of HSP90-CDC37-BRAFV600E complexes with PP5, in autoinhibited and activated conformations, together with proteomic analysis of its phosphatase activity, reveal how PP5 is activated by recruitment to HSP90 complexes to comprehensively dephosphorylate client proteins.
16
The categories of this paper are: microbiology
7
The categories of this paper are: ecology
18
The categories of this paper are: systems
8
The categories of this paper are: genomics
19
The categories of this paper are: epidemiology
27
The categories of this paper are: pathology
4
The categories of this paper are: neuroscience
12
The categories of this paper are: behavior
1
The categories of this paper are: bioinformatics
2
The categories of this paper are: biology
25
The categories of this paper are: bioengineering
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The categories of this paper are: toxicology
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The categories of this paper are: molecular
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The categories of this paper are: synthetic
6
The categories of this paper are: plant
32
The categories of this paper are: zoology