Are gene sequences used to reconstruct baterial phylogeny ?
The quickest way to identify to match an isolated strain to a species or genus today is done by amplifying it 's 16S gene with universal primers and sequence the 1.4kb amplicon and submit it to a specialised web - based identification database , namely either Ribosomal Database Projectwhich align the sequence to other 16S sequences using infernal , a secondary structure bases global alignment , or ARB SILVA , which aligns sequences via SINA ( SILVA incremental aligner ) , which does a local alignment of a seed and extends it [ http://www.arb-silva.de/documentation/background/.