Target Microorganisms | Initial Denaturation | Denaturation | Annealing | Cycles | Fw nM | Rew nM
Ejideadriuw spp. BifTOT F/BufTOT-R | 95°C,20 s | 95°C,30 s | 60°C,30 s | 40 | 200 | 300
Luctobsnffius spp. LAC-F/LAC-R | 95°C,20 s | 95°C,30 s | 63.5°C30 | 40 | 200 | 300
Becteroides fragilis group Bfra-F/Bfra-R | 95°C,20 s | 95°C,30 s | 60°C,30 s | 40 | 300 | 300
EntercbacberiaEeo-F/Eeo-R | 95°C,20 s | 95°C,30 s | 60°C,30 s | 40 | 400 | 400
Clastrifiwm cluaAer I CI-F1 /CI-F2 | 95°C,20 s | 95°C,30 s | 60°C,30 s | 40 | 200 | 200