Bayern Munich doctor Hans-Wilhelm Muller-Wohlfahrt, who has served the club for almost 40 years, has quit the Bundesliga club in the fall-out of their shock Champions League defeat by Porto. Muller-Wohlfahrt claims the German champions' medical department has been blamed for Wednesday night's 3-1 first-leg defeat in Portugal which left their Champions League hopes hanging by a thread. The 72-year-old medical professional, renowned as a world leader in his field, said 'the bond of trust is damaged' and called the blame centred at his department 'inexplicable'. His son Kilian, Peter Ueblacker and Lutz Hansel, who are all part of the team at Bayern, have also resigned. Bayern Munich doctor Hans-Wilhelm Muller-Wohlfahrt has quit  in the fall-out of their shock defeat by Porto . The 72-year-old is renowned as a world leader in his field and has been at Bayern for almost 40 years . Muller-Wohlfahrt (fourth from left) sits behind Pep Guardiola during the defeat on Wednesday evening . Hans-Wilhelm Muller-Wohlfahrt has often courted controversy with his methods. He claims that a substance called Hyalart, which is extracted from the crest of cockerels, helps lubricate knee injuries and take away pain. He also swears by Actovegin, an amino acid preparation derived from calves' blood. In 2009, he prescribed more than 50 injections of goat's blood to St Johnstone striker Peter MacDonald for his hamstring injury. Among those to have been treated by him are Ronaldo, Usain Bolt, Michael Owen and Jose Maria Olazabal. Muller-Wohlfahrt claims to have administered more than one million injections, at least half of which have been to athletes. A statement from Muller-Wohlfahrt read: 'After the Champions League match of Bayern Munich against Porto the medical department was for some inexplicable reason made primarily responsible. The bond of trust is damaged.' And on Friday morning, the club released the following statement: 'FC Bayern Munich notes with regret the decision of chief medical officer Dr Hans-Wilhelm Müller-Wohlfahrt (72) to resign his medical duties at the club. 'Dr Muller-Wohlfahrt in particular, but also his team, have performed first-class work for the club and its players over the years. Our sincere thanks are due for this. 'FC Bayern will shortly announce new arrangements for the club’s medical unit. Until then Dr Volker Braun, sports physician and consulting physician in trauma surgery and orthopaedics, will support the FC Bayern team at matches.' Pep Guardiola had just 14 fit players to choose from at the Estadio do Dragao and the Bayern manager implied in his post-match comments that the defeat was down to the lack of available players. 'We have players who were out a long time with injuries,' he said. 'Their legs don't last very long. It's a tough result to take into the second leg, but we'll give it a go.' Muller-Wohlfahrt comes to Bayern Munich's Arjen Robben during a Bundesliga game last month . Guardiola failed to win the Champions League in his first season in Germany and is on the brink of exit again . Speaking at a press conference on Friday, Guardiola said: 'It was his decision to leave, I respect his decision. That is all.' Guardiola and Muller-Wohlfahrt have reportedly had differences of opinion on several previous occasions including over the fitness of midfielder Thiago Alcantara ahead of last summer's World Cup. Mehdi Benatia, Franck Ribery, Arjen Robben, Javi Martinez and David Alaba were among those missing from the Bayern squad on Wednesday through injury. Holger Badstuber and Bastian Schweinsteiger have also struggled with long-term problems. Footage circulating online appears to show Guardiola aiming sarcastic applause at his bench against Bayer Leverkusen last week after centre back Benatia was withdrawn with an injury. Bayern say the announcement was unexpected. Media director Markus Horwick said: 'We have no knowledge of this release, so we can not comment.' Usain Bolt was one of many athletes to benefit from Muller-Wohlfahrt's treatment at the London Olympics . Muller-Wohlfahrt has worked with the German national team as well as athletes including Usain Bolt and Boris Becker. The Bayern fall-out will not end his work with Joachim Low's team. 'He is the best doctor in the world,' Bolt said after being treated by Muller-Wohlfahrt at the London Olympics in 2012. 'The Doctor is a very, very great man. Thank you, Doctor!' He previously quit Bayern after falling out with then-coach Jurgen Klinsmann in the 2008-09 season but returned after the manager left. The German champions return to Bundesliga action on Saturday when they face Hoffenheim at the Rhein-Neckar-Arena. Usain Bolt sought the help of Hans-Wilhelm Muller-Wohlfahrt in the weeks leading up to the London Olympics in 2012. At the time, Sportsmail's Jamie Redknapp recalled his experiences with the doctor. There's not much I have in common with Usain Bolt. He's certainly a little quicker than I was at my peak. But we shared the same doctor when it came to turning to someone in need of urgent repair. When I was a Liverpool player, I regularly used the tricks and skills of Doctor Hans-Wilhelm Muller-Wohlfahrt to rescue me from another injury. Now Bolt, the greatest sprinter of all time, is visiting his Munich clinic to try and be fit for the London Olympics, which start in 18 days. I've read in these pages about Muller-Wolhfahrt's methods being described by a senior figure within the US Anti-Doping Agency as 'Frankensteintype experiments'. I'm not sure what that means, although with me he would use fluid taken from the comb on the head of a hen to lubricate a knee joint that had been eroded by a succession of injuries. He would tell me that it was like 'high quality oil for the engine of a car'. He concluded that the fluid taken from a hen's comb would lubricate the knee and allow greater movement. I was happy to do what he suggested to have the confidence to play again. I've also read about him prescribing goat's blood injections for hamstring injuries. His methods are successful, which is what has drawn Bolt around the world from his Jamaican training camp and why so many go to see the man they call 'healing Hans'. Among the sportsmen and women who have stepped into Muller-Wohlfahrt's dimly lit office are Steven Gerrard, Ronaldo, Jurgen Klinsmann, Dame Kelly Holmes, Paula Radcliffe, Michael Owen, Jose Maria Olazabal and Maurice Green. There is talk of controversy about Muller-Wohlfahrt's methods, but I won't have a bad word said against him. I saw him many times and there was never anything untoward suggested. This is not a back-street quack. He is still Bayern Munich's team doctor, world renowned and a master of his art - and they didn't seem to be doing too badly the last time I watched them in the Champions League Final. Bolt versus his Jamaican training partner, Yohan Blake, is the golden ticket for London. Last weekend, Blake beat Bolt, the fastest man on the planet, in back-to-back races over 100m and 200m. Bolt then withdrew from the Diamond League race in Monaco and the alarm bells are ringing. Nobody wants him to miss these games, surely not even Blake. Athletes generally turn to Muller-Wohlfahrt as a last act of desperation, having exhausted all the local practices and medical skills of those they have been closest to. You can't tell me that Bolt has flown all the way from Jamaica to Munich without trusting Muller-Wohlfahrt's art and without searching for a solution a little closer to home. Like me, he must trust his methods and rely on his advice. He is renowned for helping sportsmen and women, like Bolt, recover from hamstring problems. When I tore a hamstring playing for England, I was out for five months. He is getting sportsmen back on track within five weeks. I only wish I had known about him then. It appears Bolt has a back problem which may have affected his right hamstring. It doesn't sound as if Bolt has suffered a tear, more likely a tweak and if he is unable to trust his hamstrings it might explain why some of his times have been sluggish, by his standards. Didi Hamann, the former German international, first recommended Muller- Wohlfahrt to me when I didn't know where else to turn. I hopped on a plane with the Liverpool physio, hoping we could find some answers. When I first met him, I was anxious, but he made me feel welcome and relaxed. He was in his 60s (he's 70 next month), but he had the skin of a man 20 years younger, with a shock of hair and healthy outlook on life. He believes in the power of positive thinking, he is a charismatic man who convinces you he can find the answers. Most doctors do, but he backs it up with achieving success. His staff are dressed from head to toe in white and he is there, overseeing the treatments and working from 7am-11pm, trying to find the solutions for a conveyor belt of people, including the general public, who come looking for answers. My club, Liverpool, paid for my treatment through insurance, but when I bumped into Hans at a recent Champions League game, he asked me how my knee was and I told him it was still causing me hassle. He told me to come and see him and after some treatment I asked him where I could pay the bill. He said: 'There isn't one, Jamie.' It was a kind gesture from a very skilled man. Bolt is in Safe Hands. Muller-Wohlfahrt has worked with the German national team as well as athletes including Usain Bolt .
Bayern Munich's long-serving doctor Hans-Wilhelm Muller-Wohlfahrt quits.
German champions lost 3-1 in the Champions League to Porto this week.
Muller-Wohlfahrt claimed he and his staff were blamed for the defeat.
Bayern release statement on Friday thanking Muller-Wohlfahrt for his services and saying they 'regret' his decision.
Guardiola on Friday said he 'respects' Muller-Wohlfahrt's decision.
Pep Guardiola seemed to place blame on injuries in post-match interview.