Physico-chemical properties of interferon produced by a mixed leukocyte suspension.
Physico-chemical properties of partially purified interferon produced by a mixed culture of human peripheral blood leukocytes following induction with double-stranded RNA extracted from f2 phage infected Escherichia coli were studied. Molecular heterogeneity of the interferon preparation was demonstrated by gel chromatography on a Sephadex G-100 column, disc electrophoresis in polyacrylamide gel and by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The first two methods revealed 5 peaks, the latter 7 peaks of interferon activity. There was no difference in the molecular nature of interferon produced by cultures exposed to the inducer for the whole period of incubation and that produced by cultures from which the inducer was removed after a short induction time.
Physico-chemical properties of interferon produced by a mixed leukocyte suspension. Physico-chemical properties of partially purified interferon produced by a mixed culture of human peripheral blood leukocytes following induction with double-stranded RNA extracted from f2 phage infected Escherichia coli were studied. Molecular heterogeneity of the interferon preparation was demonstrated by gel chromatography on a Sephadex G-100 column, disc electrophoresis in polyacrylamide gel and by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The first two methods revealed 5 peaks, the latter 7 peaks of interferon activity. There was no difference in the molecular nature of interferon produced by cultures exposed to the inducer for the whole period of incubation and that produced by cultures from which the inducer was removed after a short induction time.