Gaucher's disease. Factors affecting the 4-methylum-belliferyl-beta-D-glucosidase activity of cultured skin fibroblasts.
Phosphate-buffered saline was found to inhibit acid beta-glucosidase activity of cultured cells. This effect was attributable to both the pH and the chloride content of the phosphate-buffered saline. Washing the cells in sucrose (0.25M) to remove residual phosphate-buffered saline effectively eliminated this inhibition.
Gaucher's disease. Factors affecting the 4-methylum-belliferyl-beta-D-glucosidase activity of cultured skin fibroblasts. Phosphate-buffered saline was found to inhibit acid beta-glucosidase activity of cultured cells. This effect was attributable to both the pH and the chloride content of the phosphate-buffered saline. Washing the cells in sucrose (0.25M) to remove residual phosphate-buffered saline effectively eliminated this inhibition.