[Study of the biosynthesis of a carbohydrate fragment of rubomycin].
When the rubomycin-producing organism was grown in the presence of an evenly labeled C14-glucose and various non-labeled carbon sources, the carbohydrate part of the antibiotic also became evently labeled. The activity of I carbon atom of rubomycin sugar grown against the background of non-labeled glycerol was almost 3 times higher than the respective value of the aglycone activity. C14-acetate, C14-propionate, C14-methionine and daunosamine were not incorporated. The data are indicative of the fact that there was no splitting of the glucose carbon skeleton during conversion of glucose into amino sugar.
[Study of the biosynthesis of a carbohydrate fragment of rubomycin]. When the rubomycin-producing organism was grown in the presence of an evenly labeled C14-glucose and various non-labeled carbon sources, the carbohydrate part of the antibiotic also became evently labeled. The activity of I carbon atom of rubomycin sugar grown against the background of non-labeled glycerol was almost 3 times higher than the respective value of the aglycone activity. C14-acetate, C14-propionate, C14-methionine and daunosamine were not incorporated. The data are indicative of the fact that there was no splitting of the glucose carbon skeleton during conversion of glucose into amino sugar.