Improved methods of influenza virus propagation. I. Enhancement of virus reproduction in cell cultures.
Comparative studies on the reproduction of a set of influenza A and B virus strains in different cell cultures were carried out. A cultivation system yielding influenza virus with high infectivity, based on the use of roller cultures of bovine embryo kidney cells, of virus strains adapted to this culture, and of a maintenance medium of improved composition, was developed.
Improved methods of influenza virus propagation. I. Enhancement of virus reproduction in cell cultures. Comparative studies on the reproduction of a set of influenza A and B virus strains in different cell cultures were carried out. A cultivation system yielding influenza virus with high infectivity, based on the use of roller cultures of bovine embryo kidney cells, of virus strains adapted to this culture, and of a maintenance medium of improved composition, was developed.