Adenosine triphosphate induced change in light scattering of isolated basal bodies.
Suspensions of isolated basal bodies undergo a characteristic decrease in turbidity following their exposure to 10(-3) M ATP. Typically, turbidity changes range from 8% to 20%, depending on the preparation, with an average change of 12%. Nucleotides other than ATP did not cause a turbidity decrease. The reaction has a pH optimum of pH 8.5 and is inhibited by concentrations of divalent cations greater than 2 X 10(-3) M. These results indicate that ATP induces a conformational change in the basal body that may be related to its activity in the cell.
Adenosine triphosphate induced change in light scattering of isolated basal bodies. Suspensions of isolated basal bodies undergo a characteristic decrease in turbidity following their exposure to 10(-3) M ATP. Typically, turbidity changes range from 8% to 20%, depending on the preparation, with an average change of 12%. Nucleotides other than ATP did not cause a turbidity decrease. The reaction has a pH optimum of pH 8.5 and is inhibited by concentrations of divalent cations greater than 2 X 10(-3) M. These results indicate that ATP induces a conformational change in the basal body that may be related to its activity in the cell.