Purification and properties of hexoasmine isomerase (EC5.3.1.19) from pig intestinal mucosa.
Hexosamine isomerase from pig intestinal mucosa was purified about 70-fold. The aminosugar product of the enzymatic reaction was identified as glucosamine 6-phosphate and amino acid product as glutamic acid. The pH optimum was 7.1 Km for fructose 6-phosphate was 6.3 X 10(-4) mol/1 and for L-glutamine 6.5 X 10(-4) mol/1.
Purification and properties of hexoasmine isomerase (EC5.3.1.19) from pig intestinal mucosa. Hexosamine isomerase from pig intestinal mucosa was purified about 70-fold. The aminosugar product of the enzymatic reaction was identified as glucosamine 6-phosphate and amino acid product as glutamic acid. The pH optimum was 7.1 Km for fructose 6-phosphate was 6.3 X 10(-4) mol/1 and for L-glutamine 6.5 X 10(-4) mol/1.