Labilization of brain lysosomes by trichloroethylene metabolites and peroxide anion in vitro.
Lysosomes were isolated from adult male Wistar rat brain and incubated in vitro with 10 micron trichloroethylene and microsomes or postmicrosomal supernatant with added NADPH. Freezing and incubation with the solvent alone served as controls. The results were compared with the effect of peroxide anion from the xanthine-xanthine oxidase reaction. Mere solvent did not cause an appearance of acid proteinase activity in the incubation medium whereas other treatments induced lysosomal labilization.
Labilization of brain lysosomes by trichloroethylene metabolites and peroxide anion in vitro. Lysosomes were isolated from adult male Wistar rat brain and incubated in vitro with 10 micron trichloroethylene and microsomes or postmicrosomal supernatant with added NADPH. Freezing and incubation with the solvent alone served as controls. The results were compared with the effect of peroxide anion from the xanthine-xanthine oxidase reaction. Mere solvent did not cause an appearance of acid proteinase activity in the incubation medium whereas other treatments induced lysosomal labilization.