[Template chromatography on immobilized oligonucleotides. Synthesis and application of oligodeoxyadenosine-5'-phosphate--DEAE-cellulose (author's transl)].
Oligomers of deoxyadenylic acid, obtained by polycondensation, were covalently attached to polyvinyl alcohol. These polymer-bound oligonucleotides undergo very strong adsorption on DEAE-cellulose such that at neutral pH and with 1 M NaCl, partial desorbtion occurs only above 60 degrees. The use of this PV(pA)n-DEAE-cellulose for the column chromatographic separation of deoxythymidylic acid oligomers obtained by polycondensation, according to the principle of base-pairing, is discussed. Linear oligomers and also pyrophosphate derivatives of thymidylic acid which contain more than five monomer units undergo strong retardation under the conditions of base-pairing. Cyclic oligonucleotides do not show any noticeable interaction with the stationary phase. Thus, through the use of a temperature gradient, it is possible to fractionally separate the polycondensate, giving an average degree of polymerisation of 6--10.
[Template chromatography on immobilized oligonucleotides. Synthesis and application of oligodeoxyadenosine-5'-phosphate--DEAE-cellulose (author's transl)]. Oligomers of deoxyadenylic acid, obtained by polycondensation, were covalently attached to polyvinyl alcohol. These polymer-bound oligonucleotides undergo very strong adsorption on DEAE-cellulose such that at neutral pH and with 1 M NaCl, partial desorbtion occurs only above 60 degrees. The use of this PV(pA)n-DEAE-cellulose for the column chromatographic separation of deoxythymidylic acid oligomers obtained by polycondensation, according to the principle of base-pairing, is discussed. Linear oligomers and also pyrophosphate derivatives of thymidylic acid which contain more than five monomer units undergo strong retardation under the conditions of base-pairing. Cyclic oligonucleotides do not show any noticeable interaction with the stationary phase. Thus, through the use of a temperature gradient, it is possible to fractionally separate the polycondensate, giving an average degree of polymerisation of 6--10.