Immunoreactive properties of anti-thyroglobulin autoantibodies isolated by affinity chromatography from human thyroiditis serum.
A Sepharose-coupled 19S human thyroglobulin has been used as an immunoadsorbent to isolate anti-thyroglobulin autoantibodies and to evaluate the antigen-antibody interactions. With the system proposed a high yield of active antibody molecules was obtained. It is possible to evaluate both the soluble and precipitating 'immunological interactions', thus avoiding the use of the double antibody technique.
Immunoreactive properties of anti-thyroglobulin autoantibodies isolated by affinity chromatography from human thyroiditis serum. A Sepharose-coupled 19S human thyroglobulin has been used as an immunoadsorbent to isolate anti-thyroglobulin autoantibodies and to evaluate the antigen-antibody interactions. With the system proposed a high yield of active antibody molecules was obtained. It is possible to evaluate both the soluble and precipitating 'immunological interactions', thus avoiding the use of the double antibody technique.