[Oxidative metabolism of funicular tissue. II. Determination of the activity of certain Krebs cycle enzymes].
Previous experiments have suggested that a partial metabolic block might restrain the oxidative metabolism of the cord tissue between the decarboxylation of pyruvate and the oxidation of succinate. Some of the dehydrogenases of the Kreb's cycle were assayed on acetone powders prepared from human cords. Isocitrate dehydrogenases (both NAD and NADP-specific) have much lower activities than the alpha-ketoglutarate- and malate dehydrogenases; a partial block might be located at this level. Moreover, the malic enzyme has a rather high activity, and might play a significant role by regenerating NADPH in a tissue where other sources of this coenzyme are practically absent.
[Oxidative metabolism of funicular tissue. II. Determination of the activity of certain Krebs cycle enzymes]. Previous experiments have suggested that a partial metabolic block might restrain the oxidative metabolism of the cord tissue between the decarboxylation of pyruvate and the oxidation of succinate. Some of the dehydrogenases of the Kreb's cycle were assayed on acetone powders prepared from human cords. Isocitrate dehydrogenases (both NAD and NADP-specific) have much lower activities than the alpha-ketoglutarate- and malate dehydrogenases; a partial block might be located at this level. Moreover, the malic enzyme has a rather high activity, and might play a significant role by regenerating NADPH in a tissue where other sources of this coenzyme are practically absent.