Isolation and properties of muscular glucose dehydrogenase (E.C. 1.1.1.47) from the stomachs of geese and ducks.
A method which purified 30-fold perparations of muscular glucose dehydrogenase from the stomachs of geese and ducks was elaborated. Activity of this enzyme was found to be dependent on presence of free-SH groups, NAD or NADP as coenzymes, and had an optimum at about pH 7-8. The enzyme's activity against 17 different monosaccharides and their derivatives was tested. The best substrate was beta-D-glucose, followed by D-glucose, D-glucosamine and D-xylose. Other compounds such as D-glucose-6-phosphate, D-fructose, D-mannose and 2-deoxyglucose were not oxidized. The enzyme preparation showed presence of enzymes which conduct gluconic acid metabolism.
Isolation and properties of muscular glucose dehydrogenase (E.C. 1.1.1.47) from the stomachs of geese and ducks. A method which purified 30-fold perparations of muscular glucose dehydrogenase from the stomachs of geese and ducks was elaborated. Activity of this enzyme was found to be dependent on presence of free-SH groups, NAD or NADP as coenzymes, and had an optimum at about pH 7-8. The enzyme's activity against 17 different monosaccharides and their derivatives was tested. The best substrate was beta-D-glucose, followed by D-glucose, D-glucosamine and D-xylose. Other compounds such as D-glucose-6-phosphate, D-fructose, D-mannose and 2-deoxyglucose were not oxidized. The enzyme preparation showed presence of enzymes which conduct gluconic acid metabolism.