Splenic foci of cytotoxic lymphocytes in a graft-versus-host reaction.
It has been established that cytotoxic lymphocytes are generated in discrete foci in the spleens of mice undergoing a graft-versus-host reaction. The foci segregated randomly in such spleens. 2.8 X 10(4) lymph node cells, 2.3 X 10(5) spleen cells and 2.7 X 10(6) thymus cells from CBA mice generated a mean of one focus in lethally irradiated (CBA X DBA)F1 recipient mice. Cytotoxic foci were detected when cytotoxicity was measured by two different cytotoxicity assays, the release of [51Cr]-sodium chromate from [51Cr]-labelled target cells and colony inhibition. The seeding efficiency of cytotoxic focus-forming cells has been estimated at 19%.
Splenic foci of cytotoxic lymphocytes in a graft-versus-host reaction. It has been established that cytotoxic lymphocytes are generated in discrete foci in the spleens of mice undergoing a graft-versus-host reaction. The foci segregated randomly in such spleens. 2.8 X 10(4) lymph node cells, 2.3 X 10(5) spleen cells and 2.7 X 10(6) thymus cells from CBA mice generated a mean of one focus in lethally irradiated (CBA X DBA)F1 recipient mice. Cytotoxic foci were detected when cytotoxicity was measured by two different cytotoxicity assays, the release of [51Cr]-sodium chromate from [51Cr]-labelled target cells and colony inhibition. The seeding efficiency of cytotoxic focus-forming cells has been estimated at 19%.