Regulation of glnA messinger ribonucleic acid synthesis in Klebsiella aerogenes.
We examined wild-type and mutant strains of Klebsiella aerogenes for the relative amounts of ribonucleic acid (RNA) hybridizing specifically to deoxyribonucleic acid from a transducing phage carrying glnAK, the structural gene for glutamine synthetase. Our data showed a positive correlation between the intracellular level of glutamine synthetase and the level of glnA messenger RNA; we were unable to detect glnA messinger RNA in strains devoid of glutamine synthetase protein. Therefore, it is possible that transcription of glnA is not regulated simply by repression mediated through the glutamine synthetase protein; rather, autogenous control in this system may involve activation of transcription. Our experiments also suggest that the promotor of the glnA gene is located at the rha proximal end of the gene.
Regulation of glnA messinger ribonucleic acid synthesis in Klebsiella aerogenes. We examined wild-type and mutant strains of Klebsiella aerogenes for the relative amounts of ribonucleic acid (RNA) hybridizing specifically to deoxyribonucleic acid from a transducing phage carrying glnAK, the structural gene for glutamine synthetase. Our data showed a positive correlation between the intracellular level of glutamine synthetase and the level of glnA messenger RNA; we were unable to detect glnA messinger RNA in strains devoid of glutamine synthetase protein. Therefore, it is possible that transcription of glnA is not regulated simply by repression mediated through the glutamine synthetase protein; rather, autogenous control in this system may involve activation of transcription. Our experiments also suggest that the promotor of the glnA gene is located at the rha proximal end of the gene.