Factors affecting the assay of gentamicin by the plate diffusion method.
Standard curves were prepared by plotting log gentamicin concentration against zone diameter using a conventional plate diffusion method. Results were obtained at varying concentrations of sodium chloride and at different pHs. Under optimum conditions the range in zone diameters was markedly increased, thus considerably improving the potential accuracy of the plate assay method.
Factors affecting the assay of gentamicin by the plate diffusion method. Standard curves were prepared by plotting log gentamicin concentration against zone diameter using a conventional plate diffusion method. Results were obtained at varying concentrations of sodium chloride and at different pHs. Under optimum conditions the range in zone diameters was markedly increased, thus considerably improving the potential accuracy of the plate assay method.