[Study of acetyl-CoA-synthetase from staphylococcus aureus].
Acetyl-CoA-synthetase was isolated from cells of St. aureus 209-P. The method of isolation and partial purification of the enzyme is worked out. Km values of the enzyme for acetate, CoA and ATP are calculated. p-Chloromercuribenzoate and monoiodoacetate were shown to inhibit the enzyme activity. The enzyme activity is estimated depending on the age of the cell culture and on the presence of acetate in the culture medium.
[Study of acetyl-CoA-synthetase from staphylococcus aureus]. Acetyl-CoA-synthetase was isolated from cells of St. aureus 209-P. The method of isolation and partial purification of the enzyme is worked out. Km values of the enzyme for acetate, CoA and ATP are calculated. p-Chloromercuribenzoate and monoiodoacetate were shown to inhibit the enzyme activity. The enzyme activity is estimated depending on the age of the cell culture and on the presence of acetate in the culture medium.