Ultraviolet spectrometry of serum triglycerides by a totally enzymic method adapted to a centrifugal analyzer.
We described a micromethod for determining serum triglycerides (triacylglycerols) with the centrifugal analyzer. This technique is based on the procedure of Bublitz and Kennedy [J. Biol. Chem. 211, 951 (1954)]. The enzymic hydrolysis requires 10 min at 30 degrees C. Twenty-six serum triglyceride assays can be done in about 30 min. Concentration and absorbance are linearly related up to 5.0 mmol/liter, but higher concentrations can be assayed by changing conditions slightly. Day-to-day reproducibility for the method was satisfactory (CV, 2.7 to 8.4%). Comparison of this method and two other methods for triglycerides, the automated Hantzsch condensation method and a commercial enzymic method (Calbiochem), gave correlation coefficients of 0.976 and 0.990, respectively. Results are unaffected by the presence of endogenous serum ADP, pyruvic acid, phosphatases, or ATPase.
Ultraviolet spectrometry of serum triglycerides by a totally enzymic method adapted to a centrifugal analyzer. We described a micromethod for determining serum triglycerides (triacylglycerols) with the centrifugal analyzer. This technique is based on the procedure of Bublitz and Kennedy [J. Biol. Chem. 211, 951 (1954)]. The enzymic hydrolysis requires 10 min at 30 degrees C. Twenty-six serum triglyceride assays can be done in about 30 min. Concentration and absorbance are linearly related up to 5.0 mmol/liter, but higher concentrations can be assayed by changing conditions slightly. Day-to-day reproducibility for the method was satisfactory (CV, 2.7 to 8.4%). Comparison of this method and two other methods for triglycerides, the automated Hantzsch condensation method and a commercial enzymic method (Calbiochem), gave correlation coefficients of 0.976 and 0.990, respectively. Results are unaffected by the presence of endogenous serum ADP, pyruvic acid, phosphatases, or ATPase.