Binding of nuclear triiodothyronine (T3) binding protein-T3 complex to chromatin.
Nuclear tirrodothyronine (T3) binding protein (NTBP)-T3 complex, prepared from liver nuclei of rats given [125I]T3 in vovo, rebinds to rat nuclear chromatin at pH 7.4 and at low, but not high, KCl concentrations. Liver NTBP-T3 complex binds to chromatin from liver, kidney, heart, brain, testis, and spleen. Binding was depressed at pH8, by addition of 10 mm CaCl2 or 100 mM MgCl2, and by 1 mM GTP or UTP. Although heart chromatin bound the most NTBP-T3 complex and brain the least, there is no clear separation of binding activity on comparison of three T3-responsive tissues (heart, liver, kidney) to the T3 insensitive tissues. Under the conditions of these experiments, there was no evident competition for binding sites on any of the six chromatins tested.
Binding of nuclear triiodothyronine (T3) binding protein-T3 complex to chromatin. Nuclear tirrodothyronine (T3) binding protein (NTBP)-T3 complex, prepared from liver nuclei of rats given [125I]T3 in vovo, rebinds to rat nuclear chromatin at pH 7.4 and at low, but not high, KCl concentrations. Liver NTBP-T3 complex binds to chromatin from liver, kidney, heart, brain, testis, and spleen. Binding was depressed at pH8, by addition of 10 mm CaCl2 or 100 mM MgCl2, and by 1 mM GTP or UTP. Although heart chromatin bound the most NTBP-T3 complex and brain the least, there is no clear separation of binding activity on comparison of three T3-responsive tissues (heart, liver, kidney) to the T3 insensitive tissues. Under the conditions of these experiments, there was no evident competition for binding sites on any of the six chromatins tested.