Definition of period of induction of deciduoma in the rat using ornithine decarboxylase as a marker of growth onset.
The activity of uterine ornithine decarboxylase (ODC) was measured during the 24 hours after systemic induction of decidualization. Following a latent period of 2.5 hours, activity rose and reached a peak of 5.6 +/- 1.1 fold at 5 hours after induction. The activity then declined and a second, lower peak was seen at 21 hours. The increase in ODC activity was suppressed by treatment with cycloheximide (50 mg/kg) and dactinomycin, 500 microgram/rat, confirming that the enzyme response reflects protein synthesis. The increase and the base-line level of ODC activity were suppressed by ergocornine pretreatment (1 mg/rat) which disturbs the endocrine balance of pseudopregnancy. It was concluded that the growth and differentiation of decidual tissue began 2.5 hours after application of the induction stimulus.
Definition of period of induction of deciduoma in the rat using ornithine decarboxylase as a marker of growth onset. The activity of uterine ornithine decarboxylase (ODC) was measured during the 24 hours after systemic induction of decidualization. Following a latent period of 2.5 hours, activity rose and reached a peak of 5.6 +/- 1.1 fold at 5 hours after induction. The activity then declined and a second, lower peak was seen at 21 hours. The increase in ODC activity was suppressed by treatment with cycloheximide (50 mg/kg) and dactinomycin, 500 microgram/rat, confirming that the enzyme response reflects protein synthesis. The increase and the base-line level of ODC activity were suppressed by ergocornine pretreatment (1 mg/rat) which disturbs the endocrine balance of pseudopregnancy. It was concluded that the growth and differentiation of decidual tissue began 2.5 hours after application of the induction stimulus.